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Green Sky Labs xanthohumol pure extract xn
XN and HOP EX affect HNSCC cell viability: ( A ) <t>Xanthohumol</t> chemical structure. ( B ) Viability assay measured by MTT (concentration range 0–25 µM XN, 0–1% HOP EX) at 48 h post-treatment. ( C ) Apoptosis analysis by Annexin V/PI assay 48 h post-treatment with IC50 (original plot Fig. C) ( D ) Synergyfinder + analysis shows significant synergism in combinations of XN + Cisplatin in UMSCC-103 and CAL-33. ( E ) Western blot results and quantification of BAX/BCL2 ratio in XN, Cisplatin, and combination treatment compared to the control. ( F ) Synergyfinder + analysis shows significant synergism in combinations of XN + Cisplatin in UMSCC-103 cis-R. ( G ) XN sensitivity (MTT assay) of UMSCC-103 cis-R cells.
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XN and HOP EX affect HNSCC cell viability: ( A ) Xanthohumol chemical structure. ( B ) Viability assay measured by MTT (concentration range 0–25 µM XN, 0–1% HOP EX) at 48 h post-treatment. ( C ) Apoptosis analysis by Annexin V/PI assay 48 h post-treatment with IC50 (original plot Fig. C) ( D ) Synergyfinder + analysis shows significant synergism in combinations of XN + Cisplatin in UMSCC-103 and CAL-33. ( E ) Western blot results and quantification of BAX/BCL2 ratio in XN, Cisplatin, and combination treatment compared to the control. ( F ) Synergyfinder + analysis shows significant synergism in combinations of XN + Cisplatin in UMSCC-103 cis-R. ( G ) XN sensitivity (MTT assay) of UMSCC-103 cis-R cells.

Journal: Scientific Reports

Article Title: Xanthohumol modulate autophagy and ER stress to counteract stemness and enhance cisplatin efficacy in head and neck squamous cell carcinoma

doi: 10.1038/s41598-025-98003-1

Figure Lengend Snippet: XN and HOP EX affect HNSCC cell viability: ( A ) Xanthohumol chemical structure. ( B ) Viability assay measured by MTT (concentration range 0–25 µM XN, 0–1% HOP EX) at 48 h post-treatment. ( C ) Apoptosis analysis by Annexin V/PI assay 48 h post-treatment with IC50 (original plot Fig. C) ( D ) Synergyfinder + analysis shows significant synergism in combinations of XN + Cisplatin in UMSCC-103 and CAL-33. ( E ) Western blot results and quantification of BAX/BCL2 ratio in XN, Cisplatin, and combination treatment compared to the control. ( F ) Synergyfinder + analysis shows significant synergism in combinations of XN + Cisplatin in UMSCC-103 cis-R. ( G ) XN sensitivity (MTT assay) of UMSCC-103 cis-R cells.

Article Snippet: Xanthohumol pure extract (XN) and Xanthohumol Hop extract 2% purity (HOP-EX) were obtained from Greensky.

Techniques: Viability Assay, Concentration Assay, Western Blot, Control, MTT Assay